OSE Seminar by Dr. Diane Lidke on Fluorescence Nanoscopy to Study Cell Siganling

Departmental News

Dr. Diane Lidke 225x300

Posted: April 18, 2023


Dr. Diane S. Lidke
Pathology Department
University of New Mexico

Date: Thursday, April 20, 2023

Time:  12:15 PM - 1:15 PM

Location: PAIS, Room 2540.

Complex cellular processes are governed by signal transduction, which in turn is controlled by protein-protein interactions at the plasma membrane and along the signaling cascade. Fluorescence microscopes are now becoming fluorescence “nanoscopes” as single molecule and super-resolution imaging go beyond the diffraction limit, enabling the quantification of protein dynamics and distribution in intact cells. This seminar will focus on how we apply these techniques in innovative ways to determine the influence of protein interactions in controlling cellular outcomes in both immune cells and cancer.



My research integrates the disciplines of biophysics, bio-imaging and quantitative biology to gain new and fundamental understanding of the components and dynamics of cell signaling pathways. This involves the measurement of protein behavior in living cells to capture and quantify biochemical events that initiate signaling. My lab focuses on the study of immunoreceptors and the EGFR/ErbB family of receptor tyrosine kinases, and how these are altered in diseased cells or influenced by therapeutics.

I have a strong track-record of developing innovative ways to measure biological processes and was a recipient of the 2011 Biophysical Society’s Margaret Oakley Dayhoff Award for my “innovative work integrating the disciplines of biophysics, bioimaging and quantitative biology.” My expertise in live cell imaging includes techniques such as FRAP, FCS, FRET, hyperspectral imaging, super-resolution microscopy and single particle tracking, as well as development of techniques for live cell labeling of proteins of interest with fluorescent proteins and/or quantum dot probes [1-4]. I am the Scientific Director of the UNM CCC Fluorescence Microscopy Shared Resource